Purification and Concentration of Enterokinase
نویسنده
چکیده
A concentrated solution of purified enterokinase is conveniently prepared from the fluid contents of pigs' duodena by means of fractional precipitation with ammonium sulfate under the proper pH conditions.
منابع مشابه
Purification of porcine enterokinase by affinity chromatography.
A method is described for the purification of porcine enterokinase by affinity chromatography with p-aminobenzamidine as the ligand. Purification was completed by immunoadsorption with antisera raised to components binding non-biologically to the gel. The final enterokinase preparation was 2.3 times more active than the most active preparation previously described.
متن کاملExpression and Purification of Soluble form of Human Parathyroid Hormone (rhPTH1-34) by Trx Tag in E. coli
Background: Parathyroid Hormone (PTH) is secreted by parathyroid glands and controls the level of calcium in bones and kidney. PTH is a small polypeptide with 84 amino acids, but the first 34 amino acids of which are enough for hormone biological activity and can be used in the treatment of Osteoporosis. The expression efficiency of recombinant human parathyroid hormone rhPTH (1-34) or Teripara...
متن کاملFormation of Trypsin from Crystalline Trypsinogen by Means of Enterokinase
Crystalline trypsinogen is most readily and completely transformed into trypsin by means of enterokinase in the range of pH 5.2-6.0 at 5 degrees C. and at a concentration of trypsinogen of not more than 0.1 mg. per ml. The action of enterokinase under these conditions is that of a typical enzyme. The process follows closely the course of a catalytic unimolecular reaction, the rate of formation ...
متن کاملHigh-level Production of Bovine Enterokinase Light Chain Using Fed-batches by Recombinant Pichia pastoris
A recombinant Pichia pastoris containing enterokinase light chain gene from bovine was cultured in flasks firstly at different pH, methanol addition, and cell mass concentration for enterokinase production. Activity of enterokinase increased as cell mass concentration increased at more than = 6 % methanol added. No significant change of activity of enterokinase was observed when methanol added ...
متن کاملSimple purification of a foreign protein using polyhedrin fusion in a baculovirus expression system.
Previously, we found that expression by translational fusion of the polyhedrin (Polh)-green fluorescence protein (GFP) led to the formation of granular structures, and that these fluorescent granules were easily precipitated by high-speed centrifugation. Here, we developed an easy, fast, mass purification system using this baculovirus expression system (BES). An enhanced GFP (EGFP) fused with t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of General Physiology
دوره 22 شماره
صفحات -
تاریخ انتشار 1939